Ultra-deep sequencing reveals no evidence of oncogenic mutations or enrichment by ex vivo CRISPR/Cas9 genome editing in human hematopoietic stem and progenitor cells
M. Kyle Cromer, Valentin V. Barsan, Erich B. Jaeger, Mengchi Wang, Jessica P. Hampton, Feng Chen, Drew Kennedy, Irina Khrebtukova, Ana Granat, Tiffany Truong, Matthew Hebden Porteus
The paper uses ultra-deep sequencing to assess oncogenic mutations in human hematopoietic stem and progenitor cells after CRISPR/Cas9 editing, finding no evidence of tumorigenic variants.
bioRxiv · Oct 27, 2021 · PreprintGenetics