Efficient Multi-Kilobase Knockins in Mice and Cell Lines using CRISPR/Cas9 and rAAV Donors with Unbiased Whole-Genome characterization by LOCK-seq
Monica F. Sentmanat, Zi Teng Wang, Evguenia V. Kouranova, Sam Peters, Wan Ching Chan, Jed Lin, Yong Miao, J. Michael White, Mia Wallace, Xiaoxia Cui
The paper develops a method for efficient multi-kilobase knock-ins in mice and cell lines using CRISPR/Cas9 and rAAV donors, and introduces LOCK-seq for comprehensive characterization of the edited genome.
Nucleic Acids Research · Mar 19, 2026Peer-reviewedGenetics