Extraction-Free Whole Transcriptome Gene Expression Analysis of FFPE Sections and Histology-Directed Subareas of Tissue
Christy L. Trejo, Miloš Babić, Elliot Imler, Migdalia Gonzalez, Sergei I. Bibikov, Peter J. Shepard, Harper C. VanSteenhouse, Joanne M. Yeakley, Bruce E. Seligmann
The paper describes a method to analyze gene expression in FFPE tissues without RNA extraction, showing high correlation with fresh samples and consistent performance across species and tissue areas.
PLoS ONE · Feb 22, 2019Peer-reviewedGenomics