Engineering a Covalent Linkage into a Dimeric De Novo Enzyme Reveals a Novel Life-Sustaining Mechanism
Guanyu Liao, Sha Tao, Mina Nagahara, Kodai Kurihara, Koji Umezawa, Ryoichi Arai, Michael H. Hecht
The paper engineers a covalent linkage into a de novo enzyme, revealing that the modified protein rescues cells through upregulating siderophore biosynthesis rather than catalyzing iron release.
bioRxiv · Dec 9, 2025 · PreprintSynthetic Biology