Single adeno-associated virus-based multiplexed CRISPR-Cas9 system to nullify core components of the mammalian molecular clock
Boil Kim, Jihoon Kim, Minjeong Chun, Inah Park, Mijung Choi, Kyungjin Kim, Han Kyoung Choe
The paper develops a CRISPR-Cas9-based AAV system to knock out multiple circadian clock genes and demonstrates its effectiveness in disrupting molecular circadian rhythms in vivo.
bioRxiv · Jul 2, 2020 · PreprintMolecular Biology