Characterization and Validation of a Novel Group of Type V, Class 2 Nucleases for in vivo Genome Editing
Matthew B. Begemann, Benjamin N. Gray, Emma January, Anna Singer, Dylan C. Kesler, Yonghua He, Haijun Liu, Hongjie Guo, Alex Jordan, Thomas P. Brutnell, Todd C. Mockler, Mohammed Oufattole
The paper identifies and validates a novel Type V, Class 2 CRISPR nuclease system (Cms1) from bacterial species that can efficiently generate indel mutations in rice.
bioRxiv · Sep 27, 2017 · PreprintMolecular Biology