Rapid and scalable characterization of CRISPR technologies using an E. coli cell-free transcription-translation system
Ryan Marshall, Colin S. Maxwell, Scott P. Collins, Thomas Jacobsen, Michelle L. Luo, Matthew B. Begemann, Benjamin N. Gray, Emma January, Anna Singer, Yonghua He, Chase L. Beisel, Vincent Noireaux
This paper introduces an E. coli cell-free system to rapidly and scalably characterize CRISPR technologies, including DNA cleavage, gene repression, and anti-CRISPR activity.
Molecular Cell · Jan 1, 2018Peer-reviewedSynthetic Biology