The importance of the location of the N-terminus in successful protein folding in vivo and in vitro
N. R. Dall, C. A. T. F. Mendonca, H. L. Torres Vera, S. Marqusee
This study investigates how circular permutation affects protein folding and aggregation in HaloTag, finding that termini placement in flexible regions is more critical than proteostasis factors.
bioRxiv · Dec 11, 2023 · PreprintBiophysics