S. Marqusee

Domain coupling in activation of a family C GPCR

This study identifies a pre-active intermediate in GPCR activation and shows how dimer interactions influence signaling efficacy and functional diversity.

The importance of the location of the N-terminus in successful protein folding in vivo and in vitro

This study investigates how circular permutation affects protein folding and aggregation in HaloTag, finding that termini placement in flexible regions is more critical than proteostasis factors.

Step-wise activation of a Family C GPCR

The paper proposes a step-wise activation model for metabotropic glutamate receptor subtype 5, supported by structural and fluorescence imaging data of different activation states.

The importance of input sequence set to consensus-derived proteins and their relationship to reconstructed ancestral proteins

The paper compares consensus and ancestral protein reconstruction methods using Ribonuclease H sequences, finding that phylogenetically restricted consensus proteins are more stable than those from diverse clades.

Nanopore molecular trajectories of a eukaryotic reverse transcriptase reveal a long-range RNA structure sensing mechanism

The paper uses nanopore sequencing to study the single-nucleotide kinetics of a reverse transcriptase, revealing its ability to sense and destabilize RNA structures during cDNA synthesis.

Identification of a conserved neutralizing epitope present on spike proteins from all highly pathogenic coronaviruses

The paper identifies a conserved neutralizing epitope on the S2 domain of SARS-CoV, SARS-CoV-2, and MERS-CoV spike proteins that is targeted by antibody 3A3.

Ultrapotent SARS coronavirus-neutralizing single-domain antibodies that bind a conserved membrane proximal epitope of the spike

The paper identifies ultrapotent SARS-CoV-2-neutralizing nanobodies that bind a conserved epitope on the spike protein, showing therapeutic efficacy in hamsters.

A central helical fulcrum in eIF2B coordinates allosteric regulation of Integrated Stress Response signaling

The paper identifies a Switch-Helix in eIF2B that allosterically regulates ISR signaling and is conserved across species.

Negative allosteric modulation of the glucagon receptor by RAMP2

This study shows RAMP2 inhibits GCGR signaling by inducing conformational changes and disorder in the receptor's extracellular domain.

Evolution avoids a pathological stabilizing interaction in the immune protein S100A9

The paper identifies a mutation in S100A9 that stabilizes a non-functional conformation, revealing an evolutionary constraint on stabilizing mutations.

Saturation mutagenesis of a predicted ancestral Syk-family kinase

The paper generates a bacterial-expressible Syk-family kinase via ancestral sequence reconstruction and uses deep mutational scanning to identify functional mutations.

A Saturation-Mutagenesis Analysis of the Interplay Between Stability and Activation in Ras

This study shows that cancer hotspot mutations in Ras activate it regardless of stability, while non-hotspot mutations can be activating or deleterious depending on stability.

Ligand-induced changes in dynamics mediate long-range allostery in the lac repressor

This study uses HDX/MS to show ligand-induced changes in dynamics mediate long-range allostery in the lac repressor, revealing flexibility differences in secondary structures across functional states.

Rigid DNA nanotube tethers suppress high frequency noise in dual-trap optical tweezers systems

This study evaluates how rigid DNA nanotube tethers affect noise in dual-trap optical tweezers systems, finding they suppress high-frequency noise but not low-frequency noise.

Differences in the dynamics of the tandem-SH2 modules of the Syk and ZAP-70 tyrosine kinases

This study compares the conformational dynamics of the tandem-SH2 modules in Syk and ZAP-70 kinases, revealing differences in their autoinhibited states and activation mechanisms.

The SARS-CoV-2 spike reversibly samples an open-trimer conformation exposing novel epitopes

The paper identifies an alternative open-trimer conformation of the SARS-CoV-2 spike ectodomain that exposes novel epitopes and is modulated by temperature, binding, and sequence variants.

Mechanistic basis for ubiquitin modulation of a protein energy landscape

This study investigates how ubiquitination at different sites affects the energy landscape of a model protein, revealing distinct thermodynamic mechanisms for destabilization and protection.

Exploring the evolutionary history of kinetic stability in the alpha-lytic protease family

This study investigates how sequence variation in the alpha-lytic protease family relates to kinetic stability and energy landscapes, finding that pro-region size and specific amino acids influence folding speed and stability.

Single-molecule diffusometry reveals no catalysis-induced diffusion enhancement of alkaline phosphatase as proposed by FCS experiments

The paper uses single-molecule techniques to show catalysis does not enhance diffusion of alkaline phosphatase, contradicting previous FCS results attributed to substrate-induced dye blinking.

The folding and unfolding behavior of ribonuclease H on the ribosome

This study investigates how the ribosome affects the folding and unfolding of RNase H during translation, finding that ribosome-stalled RNase H unfolds faster but maintains the same folding rate.

Ubiquitination modulates a protein energy landscape site-specifically with consequences for proteasomal degradation

This study shows that ubiquitination at specific sites destabilizes protein structure and enhances proteasomal degradation, even in well-folded proteins.

α-carboxysome formation is mediated by the multivalent and disordered protein CsoS2

The paper identifies CsoS2's N-terminal peptide as a multivalent interaction hub that binds Rubisco through electrostatic interactions, enabling efficient α-carboxysome formation via avidity.

Modulating long-range energetics via helix stabilization: a case study using T4 lysozyme

This study investigates long-range energetic coupling in T4 lysozyme by analyzing a circularly permuted variant, showing that helix stabilization enhances subdomain stability through salt bridge interactions.

A small single-domain protein folds through the same pathway on- and off- the ribosome

This study shows the src SH3 domain folds through the same pathway whether on or off the ribosome, suggesting the ribosome does not alter its folding mechanism.

Tracing a proteins folding pathway over evolutionary time using ancestral sequence reconstruction and hydrogen exchange

This study uses ancestral sequence reconstruction and hydrogen exchange to trace the folding pathway of RNase H across evolutionary time, finding conserved intermediates but divergent pathways.