Repurposing CRISPR-Cas13 systems for robust mRNA trans-splicing
D. N. Fiflis, N. Rey, H. V. Lavanya, B. Sewell, A. Mitchell-Dick, S. Milo, K. Clements, A. Benkert, A. Rosales, S. Fergione, A. Asokan
This study repurposes CRISPR-Cas13 systems to enable trans-splicing of exogenous RNA fragments into endogenous pre-mRNA, improving editing of large mRNA segments in mammalian cells.
bioRxiv · Feb 6, 2024Bioengineering