Semi-quantitative detection of pseudouridine modifications and type I/II hypermodifications in human mRNAs using direct and long-read sequencing
Sepideh Tavakoli, Mohammad Nabizadeh, Amr K. Makhamreh, Howard Gamper, Caroline A. McCormick, Neda Kari Rezapour, Ya‐Ming Hou, Meni Wanunu, Sara H. Rouhanifard
The paper develops a semi-quantitative method to detect pseudouridine modifications in human mRNAs using long-read nanopore sequencing and validates it with synthetic RNA controls.
Nature Communications · Jan 19, 2023Peer-reviewedGenomics