Meni Wanunu

NanoCortex: A Unified Agentic System for Nanopore Sequencing Analysis

NanoCortex provides a unified agentic framework for nanopore sequencing analysis, improving usability over general-purpose models by integrating data processing and biological interpretation.

Rare bioactive tau oligomers from Alzheimer brain support both templated misfolding and fibril formation

The paper identifies rare bioactive tau oligomers in Alzheimer's brain that can induce templated misfolding and fibril formation, distinguishing them from non-bioactive oligomers.

Pore-Based RNA Evaluation for Control of Integrity, Sequence, and Errors - Quality Control (PRECISE-QC)

The paper introduces a quality control method for synthetic RNA using Oxford Nanopore sequencing to assess integrity, sequence accuracy, and modification sites, improving synthesis fidelity and yield.

Direct RNA sequencing of primary human T cells reveals the impact of immortalization on mRNA pseudouridine modifications.

The paper compares pseudouridine modification sites in primary and immortalized human T cells using direct RNA sequencing, finding most sites are conserved with some cell-type specific differences.

Messenger-RNA Modification Standards and Machine Learning Models Facilitate Absolute Site-Specific Pseudouridine Quantification

This paper develops machine learning models to quantitatively measure pseudouridine occupancy in human mRNAs using synthetic controls, revealing site-specific requirements for accurate classification.

mRNA psi profiling using nanopore DRS reveals cell type-specific pseudouridylation and translational regulation

The paper maps pseudouridylation sites in human cell lines using nanopore sequencing and shows how psi modifications affect translation through distinct mechanisms.

Probing enzyme-dependent pseudouridylation using direct RNA sequencing to assess neuronal epitranscriptome plasticity

This study uses direct RNA sequencing to examine pseudouridylation dynamics in neurons under healthy and unhealthy conditions, finding changes in psi writer expression and site occupancy.

Semi-quantitative detection of pseudouridine modifications and type I/II hypermodifications in human mRNAs using direct and long-read sequencing

The paper develops a semi-quantitative method to detect pseudouridine modifications in human mRNAs using long-read nanopore sequencing and validates it with synthetic RNA controls.

DNA-enhanced CuAAC ligand enables live-cell detection of intracellular biomolecules

The paper develops a DNA-enhanced CuAAC ligand for intracellular biomolecule labeling in live cells, demonstrating reduced copper toxicity and effective labeling.