Howard Gamper

This study elucidates the molecular mechanism of IF1 and IF2 in bacterial translation initiation using cryo-EM and fast-kinetics, showing their roles in subunit association, GTP hydrolysis, and Pi release.

This study investigates how E. coli recovers from growth arrest by analyzing the role of RimM in ribosome maturation and recovery mechanisms.

This study identifies a novel actinobacteria-specific ribosome tunnel occlusion factor in Mtb that blocks nascent polypeptide exit and interferes with drug binding during ribosome hibernation.

This paper develops machine learning models to quantitatively measure pseudouridine occupancy in human mRNAs using synthetic controls, revealing site-specific requirements for accurate classification.

This study uses direct RNA sequencing to examine pseudouridylation dynamics in neurons under healthy and unhealthy conditions, finding changes in psi writer expression and site occupancy.

The paper introduces Induro-tRNAseq, a method using a high-processivity reverse transcriptase to profile tRNA modifications, revealing novel proline codon modifications in humans.

This study identifies how mycobacterial Rsh detects deacylated tRNA in translating ribosomes through structural and functional analysis, revealing its constitutive surveillance mechanism.

The paper develops a semi-quantitative method to detect pseudouridine modifications in human mRNAs using long-read nanopore sequencing and validates it with synthetic RNA controls.

The paper uses time-resolved cryo-EM to visualize GTP-catalyzed ribosomal translocation by EF-G, revealing intermediate structures and mechanisms of tRNA and mRNA movement.

The paper determines the structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation using cryo-EM structures.