Validated LC-MS/MS quantification of kynurenine pathway metabolites and pathway-balance ratios in brain tissue and microglial culture supernatant: application to neonatal stroke
Md. Munnaf Hossen, Nee Edirisinghe, Emily Ross-Munro, Victor Mondal, Ritu Kumari, Abdullah Al Mamun Sohag, Isabelle K. Shearer, Bobbi Fleiss, Rosita Zakaria
The paper validates an LC-MS/MS method to quantify kynurenine pathway metabolites and ratios in brain tissue and microglial supernatant, showing altered ratios after neonatal stroke.
bioRxiv · Oct 30, 2026 · PreprintBiochemistry