A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing
D. Chandler-Brown, A. M. Bueno, O. Atay, D. S. Tsao
This paper introduces a Sanger sequencing-based COVID-19 assay that skips RNA extraction, achieving detection limits comparable to qPCR and enabling high-throughput testing.
bioRxiv · Apr 7, 2020 · PreprintMicrobiology