A quantitative model for the dynamics of target recognition and off-target rejection by the CRISPR-Cas Cascade complex
M. Rutkauskas, I. Songailiene, P. Irmisch, F. E. Kemmerich, T. Sinkunas, V. Siksnys, R. Seidel
The paper develops a quantitative model to predict CRISPR-Cas Cascade off-target recognition dynamics using single-molecule experiments and shows it accurately captures target recognition with mutations.
bioRxiv · Jan 26, 2022 · PreprintBiophysics