Stoichiometric Analysis of Protein Complexes by Cell Fusion and Single Molecule Imaging
A. Singh, A. Van Slyke, M. Sirenko, A. Song, P. Kammermeier, W. Zipfel
The paper introduces a method to quantify protein complex stoichiometry in live cells by diluting complexes in situ before single-molecule imaging, avoiding artifacts from detergent isolation or low expression levels.
bioRxiv · Jan 26, 2020Biophysics