P. Mogha

Extracellular Matrix protein gelatin provides higher expansion, reduces size heterogeneity, and maintains cell stiffness in a long-term culture of mesenchymal stem cells.

This study shows that gelatin-coated plates support better proliferation, reduced size heterogeneity, and maintained cell stiffness in long-term mesenchymal stem cell culture compared to other ECM proteins.

Soft Substrate Maintains Proliferative and Multi-lineage Potential of Human Mesenchymal Stem Cells on Long Term Expansion

This paper shows that culturing human mesenchymal stem cells on soft polyacrylamide gel maintains their stemness by delaying senescence compared to traditional tissue culture plates.