Ultrahigh-throughput directed evolution of a metal-free α/β-hydrolase with a Cys-His-Asp triad into an efficient phosphotriesterase
D. Schnettler Fernandez, O. J. Klein, T. S. Kaminski, P.-Y. Colin, F. Hollfelder
This paper evolves a metal-free α/β-hydrolase with a Cys-His-Asp triad into an efficient phosphotriesterase using ultrahigh-throughput screening, achieving catalytic efficiencies comparable to metal-dependent enzymes.
bioRxiv · Feb 14, 2022Biochemistry