O. J. Klein

Fluorogenic, sub-single-turnover monitoring of enzymatic reactions involving NAD(P)H provides a generalised platform for directed ultrahigh throughput evolution of biocatalysts in microdroplets

The paper develops a fluorogenic assay for NAD(P)H detection to enable high-throughput screening of biocatalysts in microdroplets, demonstrating improved sensitivity and applicability for glycosyl hydrolases and IREDs.

Ultrahigh-throughput directed evolution of a metal-free α/β-hydrolase with a Cys-His-Asp triad into an efficient phosphotriesterase

This paper evolves a metal-free α/β-hydrolase with a Cys-His-Asp triad into an efficient phosphotriesterase using ultrahigh-throughput screening, achieving catalytic efficiencies comparable to metal-dependent enzymes.