Insights from pooled CRISPRi single-cell screens in K562 cells reveal gene functions, regulatory networks, and highlight opportunities and limitations
Hong Zhang, Peifen Zhang, Eric Bindels, Eskeatnaf Mulugeta
This study applies a modified CRISPRi-CROP-seq protocol to knockdown six TFs in K562 cells, revealing gene functions, regulatory networks, and highlighting technical limitations of pooled CRISPRi screens.
BMC Genomics · Mar 13, 2026Peer-reviewedGenomics