Eskeatnaf Mulugeta

scRIPT is a single-cell lineage-tracing method that improves coverage and reliability in differentiation protocols by integrating rapid barcoding, sorting, and sequencing with quality control.

This study applies a modified CRISPRi-CROP-seq protocol to knockdown six TFs in K562 cells, revealing gene functions, regulatory networks, and highlighting technical limitations of pooled CRISPRi screens.

The paper investigates the roles of Atf3, Zfp711, and Bcl6b in early hemato-endothelial lineage specification using scRNA-seq and KO mESCs, finding distinct effects on mesodermal and hematopoietic lineages.

The paper identifies a novel XACT lncRNA transcript expressed in both active and inactive X chromosomes, with roles in erythroid differentiation and AML subtypes beyond its role in XCI.

The paper develops BRAIN-MAGNET, a convolutional neural network that predicts non-coding regulatory element activity and helps prioritize disease-causing variants in neurogenetic disorders.

This study perturbs LINE-1 elements in mouse embryonic stem cells to show their cis-regulatory impact on gene expression, with 1,024 genes misregulated upon activation and 81 upon repression.

The paper uses a novel method to track enhancer activity and transcriptome changes during intestinal stem cell differentiation, revealing insights into lineage decisions and chromatin dynamics.

The paper identifies 39,709 differentially active enhancers in human fetal brain development linked to neurodevelopmental disorders and GWAS loci.

The paper investigates variants in MAU2 and NIPBL in CdLS, showing NIPBL haploinsufficiency as the main cause and revealing a protective mechanism for NIPBL out-of-frame mutations.

This study identifies gene expression differences in NMR brain and gonads across reproductive castes, linking dopamine pathways and hyperprolactinaemia to eusocial reproductive suppression.