BioengineeringbioRxivPreprint
A plug-and-play system for enzyme production at commercially viable levels in fed-batch cultures of Escherichia coli BL21 (DE3)
Sujata Vijay Sohoni, Paras Harendra Kundalia, Adarsh G. Shetty…·Pramod P. Wangikar
The paper develops a fed-batch culture method for high-level enzyme production in E. coli, achieving a record nitrilase titer of 247 kU/l.
DOI 10.1101/263582