R. B. Klosgen

Preparation of physiologically active inside-out vesicles from plant inner mitochondrial membranes

This paper describes a method to isolate physiologically active inside-out vesicles from plant mitochondrial inner membranes using freeze/thaw cycles, validated by electron microscopy and enzyme activity assays.

Determining targeting specificity of nuclear-encoded organelle proteins with the self-assembling split fluorescent protein toolkit

The paper improves the sasplit-FP method to detect protein targeting specificity and develops a toolkit for high-throughput analysis of plastid and mitochondrial targeting in plants.