Engineering a seven enzyme biotransformation using mathematical modelling and characterized enzyme parts
W. Finnigan, R. Cutlan, R. Snajdrova, J. P. Adams, J. A. Littlechild, N. Harmer
The paper models and optimizes a seven-enzyme, three-step biotransformation using a deterministic process model and genetic algorithm, validated experimentally to show reliable optimization of complex enzyme cascades.
bioRxiv · Apr 9, 2019Biochemistry