R. G. Efremov

Rapid small-scale nanobody-assisted purification of ryanodine receptors for cryo-EM

This paper describes a method to rapidly purify ryanodine receptors from small protein samples for cryo-EM, demonstrating its effectiveness by solving receptor structures.

Sterol derivative binding to the orthosteric site causes conformational changes in an invertebrate Cys-loop receptor

This study presents the first cryo-EM structure of an invertebrate nACh-like receptor, revealing conformational changes induced by sterol derivative binding and unique structural features.

Time-resolved cryo-EM using a combination of droplet microfluidics with on-demand jetting

This paper develops a time-resolved cryo-EM technique with 5 ms resolution, enabling visualization of transient protein conformations in the GroEL:GroES chaperonin complex.

On a mechanistic impact of transmembrane tetramerization in pathological activation of RTKs

This paper investigates how oncogenic mutations in the transmembrane domain of RTKs promote tetramerization and ligand-independent activation through structural and dynamic changes.

AFM-based force spectroscopy unravels the stepwise-formation of a DNA transposition complex driving multi-drug resistance dissemination

The paper uses atomic force microscopy to study the stepwise formation of a DNA transposition complex involved in antibiotic resistance dissemination.

Protein compactness and interaction valency define the architecture of a biomolecular condensate across scales

This study connects molecular dynamics simulations of Lge11-80 with in vitro condensate behavior to derive a framework describing condensate architecture based on protein valency and compactness.

TCRen: predicting TCR recognition of unseen epitopes based on residue-level pairwise statistical potential

TCRen predicts TCR recognition of unseen epitopes using residue-level pairwise statistical potential from TCR-peptide contacts in crystal structures.

Structure of Escherichia coli respiratory complex I reconstituted into lipid nanodiscs reveals an uncoupled conformation

The paper presents the cryo-EM structure of E. coli complex I reconstituted in lipid nanodiscs, revealing an uncoupled conformation and proposing a new coupling mechanism.