Robust survival-based RNAi using in tandem silencing of adenine phosphoribosyltransferase
R. G. Orr, S. J. Foley, G. Galotto, B. Liu, L. Vidali
The paper develops APTi, an RNAi method in Physcomitrella patens that uses survival as a reporter to select for strongly silencing plants, achieving 93% target protein reduction.
bioRxiv · Apr 9, 2020Plant Biology