Biophysical characterization of the inactivation of E. coli transketolase by aqueous co-solvents
P. Morris, R. Garcia Arrazola, L. R. Rios Solis, P. A. Dalby
This paper investigates how polar organic co-solvents affect E. coli transketolase activity and structure, finding that solvent-induced local unfolding, not aggregation, is the main cause of enzyme inactivation.
bioRxiv · Jun 9, 2020 · PreprintBiophysics