Kinetic Interval Measurement: A Tool to Characterize Thermal Reversion Dynamics of Light-switchable Fluorescent Proteins
T. von Trotha, R. Johr, J. Fischer, L. C. Schendel, H. E. Gaub, C. Kluger
The paper introduces KIM, a method to measure thermal reversion dynamics of light-switchable proteins using a microplate reader, showing its ability to determine reversion half-life and sensitivity to temperature and imidazole concentration.
bioRxiv · Feb 20, 2021 · PreprintBiophysics