Laboratory evolution of E. coli with a natural vitamin B12 analog reveals roles for cobamide uptake and adenosylation in methionine synthase-dependent growth
K. C. Mok, Z. Hallberg, R. R. Procknow, M. E. Taga
This study evolves E. coli with a less-effective cobamide to identify genetic adaptations that improve growth, finding increased BtuB expression and BtuR overexpression enhance cobamide-dependent growth.
bioRxiv · Jan 4, 2024Microbiology