Characterizing a thermostable Cas9 for bacterial genome editing and silencing
I. Mougiakos, P. Mohanraju, E. F. Bosma, V. Vrouwe, M. Finger Bou, M. I. S. Naduthodi, A. Gussak, R. B. L. Brinkman, R. van Kranenburg, J. van der Oost
This study identifies and characterizes ThermoCas9, a thermostable Cas9 variant active across a broad temperature range, and develops tools for bacterial genome editing and silencing at elevated temperatures.
bioRxiv · Aug 18, 2017Microbiology