Generation of miR-141/200c conditional knockout mice from knockout-first, reporter-tagged parent and functional validation of the floxed allele
Sanjeev Kumar Yadav, Rashmi Srivastava, Mary-Katherine Cormier, Katie M. Lowther, Siu‐Pok Yee, Rajkumar Verma
The paper validates a two-step breeding protocol for generating miR-141/200c conditional knockout mice and shows the importance of removing the lacZ/Neo cassette for normal gene expression.
Mammalian Genome · Sep 3, 2026Peer-reviewedNeuroscience