A versatile, marker-free platform for life cycle-wide imaging in Plasmodium falciparum via integration of an exogenous gene cassette into a conserved intergenic locus
Takashi Sekine, Naoaki Shinzawa, Rie Kubota, Daisuke Kobayashi, Yawara Okubo, Kentaro Itokawa, Haruhiko Isawa, Hisako Amino, Tomoko Ishino
This paper develops a marker-free transgenic Plasmodium falciparum line with robust fluorescence across its entire life cycle, enabling live imaging and infection studies without impairing parasite growth or fitness.
bioRxiv · Jan 21, 2026 · PreprintMicrobiology