A novel platform of RNA 2'-O-methylation high-throughput and site-specific quantification tools revealed its broad distribution on mRNA
Yao Tang, Yifan Wu, Sainan Wang, Xiaolan Lu, Xiangwen Gu, Yong Li, Fan Yang, Ruilin Xu, Tao Wang, Zichen Jiao, Yan Wu, Liwei Liu, Jian‐Qun Chen, Qiang Wang, Qihan Chen
The paper introduces NJU-seq and Nm-VAQ to quantify RNA 2'-O-methylation sites in mRNA, identifying thousands of new sites and validating their presence in human and mouse cell lines.
bioRxiv · Mar 27, 2020 · PreprintGenetics