Development of a versatile high-throughput mutagenesis assay with multiplexed short read NGS using DNA-barcoded supF shuttle vector library amplified in non-SOS E. coli
Hidehiko Kawai, Ren Iwata, Shungo Ebi, Ryusei Sugihara, Shogo Masuda, Chiho Fujiwara, Shingo Kimura, Hiroyuki Kamiya
The paper develops a high-throughput mutagenesis assay using DNA-barcoded supF shuttle vectors and NGS to study mutation frequencies and spectra in E. coli.
eLife · Oct 10, 2022Peer-reviewedMolecular Biology