Expression and Purification of BsaXI Restriction Endonuclease and Engineering New Specificity from BsaXI Specificity (S) Subunit
S.-y. Xu, S. Gidwani, D. Heiter
This paper reports the cloning, expression, and engineering of BsaXI restriction endonuclease subunits to create new specificities by modifying the S subunit.
bioRxiv · Mar 14, 2022 · PreprintMicrobiology