Protocol for a minigene splice assay using the pET01 vector
Hannah Andreae, Marialessandra Curcio, Daniel Owrang, Sahar Esmaeelpour, Friederike Jahnke, Fritz Benseler, Nils Brose, Barbara Vona
The paper describes a protocol for an in vitro minigene splice assay using the pET01 vector to study aberrant splicing when patient samples or accessible tissues are unavailable.
STAR Protocols · Jun 18, 2025Peer-reviewedGenetics