Nils Brose

This study uses proximity labelling to map the proteome of different compartments in murine sensory neurons, revealing compartment-specific proteomic changes in a neuropathy model.

The paper identifies a retrograde signaling pathway where UFMylation of ER ribosomes anchors splicing factors at the ER, linking translational stress to nuclear RNA processing and altering membrane-associated gene expression.

The paper investigates the structure and function of otoferlin, showing how it binds Ca2+ and facilitates synaptic vesicle fusion in inner hair cells.

This study shows that AP2 complex is essential for myelin sheath biogenesis and homeostasis, with loss of AP2 causing hypomyelination and hypermyelination in different developmental stages.

SynapseNet automatically segments synapses in electron micrographs using deep learning, enabling efficient analysis of synaptic structures.

This study overexpresses human alpha-synuclein in rodent glutamatergic neurons and finds no significant impairment in synaptic function.

The paper describes a protocol for an in vitro minigene splice assay using the pET01 vector to study aberrant splicing when patient samples or accessible tissues are unavailable.

This paper introduces a knock-in mouse line with endogenous Munc13-1-SNAP for monitoring presynaptic nanoarchitecture using SNAP-tag labeling and live-cell imaging.

The paper introduces NbLumSyt1 nanobinders for analyzing synaptic vesicle dynamics in rodent and human models with improved precision and versatility.

The paper shows UBE3C regulates cortical neurogenesis and glial fate through m6A methylation, with mutations disrupting this process and offering therapeutic targets.

This study shows that neddylation is essential for the development and function of glutamatergic neurons, with Nedd8 deletion causing defects in dendrite complexity and synaptic transmission.

The paper uses AI classification and preprocessing to detect native SNARE and Munc13 protein bridges in cryo-ET data, revealing their role in synaptic vesicle tethering.

The paper demonstrates that presynaptic cAMP-PKA-mediated potentiation increases synaptic vesicle pool size and channel-vesicle coupling at hippocampal mossy fiber boutons.

This study uses AID2 technology to rapidly eliminate synaptic proteins and observe acute effects on receptor localization and scaffold size in neurons.

This study investigates how Neuroligin-2 interacts with MDGA1 and MDGA2 to regulate gephyrin aggregation and GABAAR function in mouse hippocampal synapses, revealing MDGA1's role in modulating these processes.

The paper uses time-lapse super-resolution microscopy to show that pre- and postsynaptic nanostructures increase in size and complexity after cLTP induction.

The paper shows that the skewed spine size distribution in neurons is not affected by synaptic activity or plasticity and forms early in neuronal development.

The paper uses cryo-electron tomography to study synaptic vesicle tethering and priming, revealing the role of Munc13 and SNAP25 in confining vesicles and guiding fusion.

This study uses single-nucleus RNA sequencing to show that erythropoietin treatment restructures transcriptional networks in hippocampal pyramidal neurons, increasing their numbers and altering gene expression related to neurodifferentiation and cognition.

This study shows that Ambra1 heterozygosity causes sex-specific reductions in excitatory synaptic activity, leading to E/I imbalance and autism-like behaviors in female mice.

The paper investigates whether Munc13 supports vesicle fusogenicity independently of active zone scaffolds and synaptic vesicle docking.

The paper identifies a two-pool mechanism in medial habenula terminals where GABAB receptor activation increases the readily releasable pool and alters vesicle coupling distances.

The paper characterizes the differential distribution and targets of Sumo paralogs in the mouse brain using knock-in mouse models and proteomic analysis.

This study identifies de novo missense variants in SLC32A1 as a cause of a neurodevelopmental disorder with epilepsy, showing they impair GABAergic neurotransmission through two mechanisms.

This study uses a fluorescent nanosensor paint to show that dopamine release from individual varicosities is highly heterogeneous and requires molecular priming.

The paper identifies key proteins and domains involved in dopamine release in the striatum, showing that RIM, Munc13, and Liprin- are essential while other active zone proteins are not.

This study investigates the roles of synaptotagmin-1 and synaptotagmin-7 in Ca2+-dependent vesicle priming and exocytosis in mouse chromaffin cells, showing their synergistic effects.

The paper investigates ultrastructural differences between Schaffer collateral and mossy fiber synapses to determine their contribution to functional synaptic heterogeneity.