Computational redesign of the Escherichia coli ribose-binding protein ligand binding pocket for 1,3-cyclohexanediol and cyclohexanol
Diogo Tavares, Artur Reimer, Shantanu Roy, Aurélie Joublin-Delavat, Vladimir Sentchilo, Jan Roelof van der Meer
The paper computationally redesigns the Escherichia coli RbsB ribose-binding protein to recognize non-natural ligands 1,3-cyclohexanediol and cyclohexanol, achieving partial success with some mutants showing ligand-specific induction.
Scientific Reports · Nov 15, 2019Peer-reviewedSynthetic Biology