APPLE-MS: A affinity purification-mass spectrometry method assisted by PafA-mediated proximity labeling
Shihan Luo, Lijuan Xie, Lin Yang, Zheyao Hu, Lei Wang, Yueqin Wang, Qingqing Li, Shujuan Guo, Sheng‐ce Tao, He‐wei Jiang
APPLE-MS improves protein-protein interaction detection by combining proximity labeling with high-specificity enrichment, revealing novel interactions and dynamics in mitochondrial and membrane-associated contexts.
bioRxiv · May 6, 2025 · PreprintBiochemistry