Cell extract-based in vitro DNA replication enables sustainable cell-free gene expression
Zheng Xiao, Wenli Gao, Wan‐Qiu Liu, Yufei Zhang, Yicong Lu, Shuhui Huang, Xiangyang Ji, Yifan Liu, Shengjie Ling, Jian Li
The paper introduces LoopReX, a cell-free gene expression system that enables sustainable DNA replication and protein production through integration of DNA replication machinery.
bioRxiv · Oct 1, 2024 · PreprintSynthetic Biology