Elucidating the Bell-Shaped Dependence of Protein Translation Activity on EF-Tu Concentration in a Reconstituted Cell-Free System Using a Mechanistic Model
Shunnosuke Ban, Yusuke Himeoka, Ako Kagawa, Yoshihiro Shimizu, Tomoaki Matsuura, Chikara Furusawa
The paper uses a mechanistic model to explain the bell-shaped protein production profile in the PURE system, showing that excess EF-Tu sequesters tRNAfMet, and confirms this by experimental validation.
ACS Synthetic Biology · May 28, 2026Peer-reviewedSynthetic Biology