Tomoaki Matsuura

This study evolves an artificial nucleocapsid from a circularly permuted bacterial protein cage, revealing heterogeneous cage architectures with distinct subunit arrangements and conformations.

This study shows that freeze-thaw cycles can concentrate molecules, form membrane compartments, and activate a transcription-translation system, suggesting a physical pathway for the emergence of functional protocells.

This paper engineers α-hemolysin to enable selective recognition, covalent linkage, and chemical exchange between artificial cells through a single protein system.

The paper uses a mechanistic model to explain the bell-shaped protein production profile in the PURE system, showing that excess EF-Tu sequesters tRNAfMet, and confirms this by experimental validation.

This paper discovers a universal polyphosphate kinase capable of phosphorylating all eight common ribonucleotides, enabling efficient in vitro transcription.

This paper introduces a protein-free method for cyclic content exchange in synthetic cells using a hydrogel-core membrane architecture.

The paper shows that phospholipid composition in icy environments influences vesicle growth and leads to selective enrichment of genetic material.

The paper generates white-eyed Daphnia magna mutants lacking scarlet function using CRISPR/Cas9 and identifies DapmaSt as a potential transformation marker.