Yoshihiro Shimizu

The paper uses a mechanistic model to explain the bell-shaped protein production profile in the PURE system, showing that excess EF-Tu sequesters tRNAfMet, and confirms this by experimental validation.

This study investigates how ZIP kinase phosphorylates smooth muscle myosin, revealing sequential phosphorylation sites and a slower rate in SMM compared to isolated MRLC due to competitive binding.

The paper analyzes over 30,000 bacterial genomes to show that CPR bacteria have fewer ribosome biogenesis factors, suggesting evolutionary flexibility in ribosome assembly.

This study develops a tRNA array method to simultaneously express all 21 tRNAs in vitro, supporting translation and genetic code engineering.

The paper develops a reconstituted system that sustainably regenerates all 20 aminoacyl-tRNA synthetases for up to 20 cycles.

This study introduces DECODE, a high-throughput method for identifying epitopes at single amino acid resolution, demonstrating its utility in antibody-based research and disease modeling.

The paper demonstrates autonomous biogenesis of the entire protein translation machinery excluding ribosomes by using surface-immobilized DNA brushes to catalyze and concentrate the synthesis of all required components.

This study develops a single-step in vitro ribosome reconstitution method using EngA and ObgE to restore ribosome activity under near-physiological conditions.

The paper introduces an improved method for multiplexed absolute protein quantification using mass spectrometry with cell-free synthesized internal standard peptides.

This paper quantitatively characterizes in silico designed riboregulators using a cell-free TX-TL system to measure their performance and dissociation constants.