Prototyping 1,4-butanediol (BDO) biosynthesis pathway in a cell-free transcription-translation (TX-TL) system
Yong Y. Wu, Stephanie Culler, Julia Khandurina, Stephen Van Dien, Richard M. Murray
This study uses a cell-free TX-TL system to prototype and optimize the 1,4-butanediol biosynthesis pathway by testing enzyme expression levels and identifying rate-limiting steps.
bioRxiv · Apr 9, 2015 · PreprintSynthetic Biology