Direct detection of mRNA expression in microbial cells by fluorescence in situ hybridization using RNase H-assisted rolling circle amplification
H. Takahashi, K. Horio, S. Kato, T. Kobori, K. Watanabe, T. Aki, Y. Nakashimada, Y. Okamura
This paper develops a FISH method to directly detect mRNA in microbial cells at the single-cell level without reverse transcription, applicable to both gram-negative and gram-positive microbes.
bioRxiv · Nov 21, 2019Microbiology