Methods Optimization for the Expression and Purification of Human Calcium Calmodulin-Dependent Protein Kinase II Alpha
S. C. Bolton, D. H. Thompson, T. L. Kinzer-Ursem
This study optimizes expression and purification protocols for human CaMKII alpha, showing insect cell systems yield higher quality autoinhibited holoenzymes than bacterial systems.
bioRxiv · Apr 28, 2023Biochemistry