Engineering a highly active thermophilic F1-ATPase by homolog-guided exploration and machine-learning-assisted prioritization
Ryohei Kobayashi, Kodai Miyake, Tatsuya Oya, Hiroshi Ueno, Yutaka Saitō, Hiroyuki Noji
This paper engineers a thermophilic F1-ATPase with higher catalytic activity using homolog-guided mutation identification and machine learning, finding a 1.8-fold increase in rotation rate.
bioRxiv · Aug 29, 2026 · PreprintBiophysics