Hiroyuki Noji

This paper engineers a thermophilic F1-ATPase with higher catalytic activity using homolog-guided mutation identification and machine learning, finding a 1.8-fold increase in rotation rate.

The paper optimizes cell-free gene expression in microdroplets to enhance protein yield from single-copy DNA templates, achieving ~94 nM expression from ~0.12 pM DNA.

The paper introduces OASSIS, an open-format digital bioassay platform that enables sensitive, low-complexity RNA detection for point-of-care testing.

The paper investigates intracellular heat dissipation mechanisms and finds that non-diffusive heat dissipation causes slow temperature relaxation in living cells compared to homogeneous solutions.

This paper investigates the role of the lower γ region in F1-ATPase rotation, finding it contributes to unidirectional rotation and torque generation specifically in the latter half of the 0-80° step.

The paper develops a bioorthogonal fluorescence probe and engineered enzyme for low-background cancer imaging in mouse models.

This paper develops a cell-free directed evolution platform using droplet microfluidics to rapidly evolve RNA polymerases with new functions and applications.

This study uses single-molecule sequencing to reveal genetic heterogeneity in influenza virus quasi-species, showing non-random mutation patterns and biological significance.

The paper characterizes the functional and structural properties of an ancestral F1-ATPase, revealing it was originally a 6-stepper that diversified into 3-, 6-, and 9-step forms.

This study uses hybrid simulations to show that side chain flexibility and angular mismatch enhance Fo motor rotation efficiency, with conserved structural features across species.

This study engineered ATP synthase to increase the H+/ATP ratio beyond natural limits, enabling ATP synthesis at lower proton motive force.

This paper develops a novel laser-induced phase separation method using the Soret effect to generate long-lasting DNA-enriched droplets, demonstrating their stability and controllability for biological applications.

This paper demonstrates self-growing artificial cell models using dextran-rich droplets in an aqueous two-phase system, coupled with DNA replication and transcription-translation systems.

This paper develops artificial cells with droplet-in-droplet structures that separate transcription and translation, mimicking eukaryotic cell compartments and enabling cascade reactions.

This study develops a rapid in vitro one-pot plasmid construction method for influenza virus synthesis, reducing plasmid construction time from days to 8 hours with comparable yield and accuracy.