Author

Hiroshi Ueno

The University of TokyoORCID 0000-0001-5331-4335Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Organism:bacteria (other)· 1 Research Focus:cell signaling & trafficking· 1 Assay:cryo-EM· 1 Approach:deep learning· 1 Assay:electronic health records· 1 Research Focus:protein design· 1 Research Focus:protein folding & dynamics· 1

Frequent Co-Authors

Hiroyuki NojiiD· 3 shared Takeshi MurataiD· 2 shared Alastair G. StewartiD· 1 shared Chihong SongiD· 1 shared Emily FurlongiD· 1 shared Kazuyoshi MurataiD· 1 shared Kiyoto Yasuda· 1 shared Kodai Miyake· 1 shared

Activity by Year

2023-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 16 mutual co-author links

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3 shared papersBiophysics

Hiroyuki NojiORCID

Mutual co-authors in cluster

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4 papers

Recent·Most cited

Engineering a highly active thermophilic F1-ATPase by homolog-guided exploration and machine-learning-assisted prioritization

Ryohei Kobayashi, Kodai Miyake, Tatsuya Oya, Hiroshi Ueno, Yutaka Saitō, Hiroyuki Noji

This paper engineers a thermophilic F1-ATPase with higher catalytic activity using homolog-guided mutation identification and machine learning, finding a 1.8-fold increase in rotation rate.

bioRxiv · Aug 29, 2026 · PreprintBiophysics

The Lower γ Region Ensures Unidirectional Rotation and Torque Generation in the Latter Half of the 80° Substep of F1-ATPase

Tomo Uchiyama, Hiroshi Ueno, Meghna Sobti, Emily Furlong, Simon H. J. Brown, Alastair G. Stewart, Hiroyuki Noji

This paper investigates the role of the lower γ region in F1-ATPase rotation, finding it contributes to unidirectional rotation and torque generation specifically in the latter half of the 0-80° step.

bioRxiv · May 12, 2026 · PreprintBiophysics

Engineering of ATP synthase for enhancement of proton-to-ATP ratio

Hiroshi Ueno, Kiyoto Yasuda, Norie Hamaguchi‐Suzuki, Riku Marui, Naruhiko Adachi, Toshiya Senda, Takeshi Murata, Hiroyuki Noji

This study engineered ATP synthase to increase the H+/ATP ratio beyond natural limits, enabling ATP synthesis at lower proton motive force.

Nature Communications · Jul 3, 2025Peer-reviewedBiophysics

Six states of Enterococcus hirae V-type ATPase reveals non-uniform rotor rotation during turnover

Raymond N. Burton‐Smith, Chihong Song, Hiroshi Ueno, Takeshi Murata, Ryota Iino, Kazuyoshi Murata

The paper identifies six states of Enterococcus hirae V-ATPase using cryo-EM, revealing non-uniform rotor rotation during turnover.

Communications Biology · Jul 28, 2023Peer-reviewedMolecular Biology